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The real-time RT-PCR was carried out in triplicates using a Roche LightCycler 480 system with a 384-well block and the software version 1.2. The 10µl reaction consisted of 25ng of transcribed RNA (1µl), 1pmol Universal Probe (0.1µl), 50pmol each of forward + reverse primer (0.5µl), water (3.4µl) and 2× LightCycler 480 Probes Master mix. The PCR-program also was used according to the manufacturer's instructions. “No template controls” and “No RT-controls” (for each cDNA-sample) showed no contamination probably affecting the results. The normalized ratio was calculated using the Relative Quantification module with the 24 hours untreated control as “Calibrator”, and the HPRT1 as reference gene resulting in individual efficiency of every single gene..

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